Disease. Gummy stem blight. Causes leaf spot, stem canker, black leaf and fruit rot; common name refers to the characteristic gummy exudate. Didymella bryoniae (Auersw.) Rehm [anamorph Phoma cucurbitacearum (Fr.) Sacc.] is the causative agent of gummy stem blight (GSB), a disease affecting. Gummy stem blight of cucurbits produces a variety of symptoms which are referred to as leaf spot, stem canker, vine wilt and black fruit rot. Lesions on leaves.
|Published (Last):||25 September 2006|
|PDF File Size:||12.77 Mb|
|ePub File Size:||19.76 Mb|
|Price:||Free* [*Free Regsitration Required]|
Mycosphaerella melonis, dudymella an ascomycete fungal plant pathogen that causes Gummy stem blight on the family Cucurbitaceae . In the current study, a credible, sensitive, and broad-spectrum diagnostic method for the detection of D. This pathogen commonly affects the foliage and stems of plants from the family Cucurbitaceae the family of gords and melonswhich includes cantaloupe, cucumber, muskmelon and watermelon plants [1,3,8].
Weeds are a source of inoculum and should be eradicated from the field before planting cucurbit crops.
There was a problem providing the content you requested
AFLP analysis of a worldwide collection of Didymella bryoniae. From native plants in central Europe to cultivated crops worldwide The emergence of Didymella bryoniae as a cucurbit pathogen.
Crop refuse should be ploughed deeply immediately after harvest to reduce fungus survival. Didymella bryoniae infects watermelon, cantaloupe, cucumber, squash, and other cucurbit hosts. On the fruit, spots appear irregularly circular and yellowish.
During the rainy season lesions can become water soaked and can spread and lead to severe defoliation. BoxArusha, Tanzania.
Spores germinate, and can directly penetrate through the cuticle or intercellular spaces and around the bases of trichomes. This improved the reaction time and efficiency. Resistance to gummy stem blight in brypniae Cucumis melo L. The disease is caused by the ascomycete Didymella bryoniae Fuckel Rehm [anamorph Phoma cucurbitacearum Fr. First report of Didymella bryoniae causing gummy stem blight of chayote in Taiwan. If these plants are transplanted in the field and survive, cankers may form on crowns or vines.
Immature pseudothecia also may form.
The asexual fruiting bodies are also flask shaped and are called pycnidia. A novel and rapid loop-mediated isothermal amplification assay for the specific detection of Verticillium dahliae.
Pale colored pycnidia are found didymlla closely spaced groups. In addition, it has been reported that the LAMP reaction might be didymelka efficient by using additional loop primers Nagamine et al. The thermal cycling program was: The method was applied to detection of D. Thus, further studies need to be carried out on a larger scale, and more field samples from other Cucurbitaceae crops in addition to muskmelon should be used to confirm the specificity of the assay.
Didymella bryoniae Fuckel Rehm, To distinguish both genotypes of D. Although very recently rapid detection of D. Loop-mediated isothermal amplification for detection of the tomato and potato late blight pathogen, Phytophthora infestans. Morphological, pathological, and genetic differentiation of Didymella bryoniae and Phoma spp. The taxonomic treatment of the genus Didymella by Corlett includes a useful summary of primary literature 6. Genomic DNA of D.
Diagnostic Guide for Gummy Stem Blight and Black Rot on Cucurbits
Genetic characterization of Didymella bryoniae isolates infecting watermelon and other cucurbits in Florida and Georgia. On pumpkin, V-shaped leaf lesions were light brown. Discussion Gummy stem fidymella caused by D. Leaf spots are tan on cucumber; brown on melon, squash, and pumpkin; and dark brown on loofah and watermelon. Gummy stem blight, caused by Didymella bryoniae, is one of the most important diseases of cucurbits in many bryoniad of the world Lee et al.
Brief review of monitoring methods for loop-mediated isothermal amplification LAMP. A polyphasic approach to characterise Phoma and related pleosporalean genera.
To control GSB of cucurbit crops, early crops with latent infection must be accurately detected and removed. An improved real-time PCR system for broad-spectrum detection of Didymella bryoniaethe causal agent of gummy stem blight of cucurbits. A set of control plants and detached leaves, mock inoculated with agar plugs, remained didymeola.
gummy stem blight of cucurbits (Didymella bryoniae)
Sexual and asexual fruiting bodies are visible on the same bryoniar of the leaves, stem, and fruits. Other diseases, such as powdery mildew Sphaerotheca fuligineamay weaken the host, making it more susceptible to D. The fruit rot phase is often called black didymekla. In addition, research not has to investigate any infected samples under greenhouse or field conditions.
As cankers expand, they become dry and rough, and the surface cracks. There were three replications for each treatment, and the experiment was repeated twice. Citron Citrullus lanatus var. Plant dis 86 —